Bolton Campylobacter Enrichment Broth 5kg

Campylobacter Enrichment Broth (Bolton Broth) is used with antimicrobics for the selective enrichment of Campylobacter spp. Campylobacter Enrichment Broth (Bolton Broth) is not intended for use in the diagnosis of disease or other conditions in humans. Campylobacter spp. are microaerophilic, very small, curved, thin, Gram-negative rods. Microaerophilic organisms have a tendency to be more sensitive to toxic forms of oxygen. Campylobacter Enrichment Broth (Bolton), along with nutritional ingredients, contains compounds which enhance the aerotolerance of microaerophilic bacteria by suppressing the toxic form of oxygen.

Preparation

1. Dissolve 27.6 g of the medium in one liter of purified water.
2. Heat with frequent agitation to completely dissolve the medium, if necessary.
3. Autoclave at 121°C for 15 minutes.
4. Cool to 45 - 50°C and aseptically add 50 mL of lysed horse blood and 2 vials of Campylobacter Supplement - Bolton (7998) or CVTN Supplement (X132).
5. Note: Blood-Free Campylobacter Enrichment Broth, Bolton’s (2X Concentration) is described by the USDA.

For specifications and data sheets please visit the documents section on the Neogen website here