Bacillus Cereus MYP Agar 500g

Bacillus Cereus MYP Agar is used with supplements for the selective and differential enumeration of Bacillus cereus from foods. Conforms to ISO and FDA / BAM formulation in a laboratory setting. MYP Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Preparation

1. Suspend 46 grams of the medium in 950 mL of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes.
4. Cool to 45 - 50°C and supplement per method below.
5. Aseptically add 50 mL of NCM4017* Egg Yolk Emulsion and 2 vials of NCM4032-0.5* Polymyxin B Supplement each reconstituted using 5 mL of sterile deionized/RO water.

Preparation per FDA BAM

1. Suspend 46 grams of the medium in 900 mL of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes.
4. Cool to 45 - 50°C and supplement per method below.
5. Aseptically add 50 mL of NCM4017* Egg Yolk Emulsion and 2 vials of NCM4032-0.5* Polymyxin B Supplement each reconstituted using 5 mL of sterile deionized/RO water.

For specifications and data sheets please visit the documents section on the Neogen website here