Violet Red Bile Agar (VRBA) (ISO) 500g

A medium for the enumeration of coliform organisms in food and dairy products according to ISO 4832:2006 and is not intended for use in the diagnosis of disease or other conditions in humans. The selectivity of the medium is due to the presence of bile salts and crystal violet. Lactose fermenters produce red/purple colonies often surrounded by a halo of the same color. Non-lactose fermenters produce pale colonies. 

Preparation

1. Suspend 38.5g of the medium in one liter of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. DO NOT AUTOCLAVE.
4. Cool to 45-50°C.

For specifications and data sheets please visit the documents section on the Neogen website here