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Description
etrathionate Broth Base is used with iodine for the recovery of Salmonella spp. in a laboratory setting. Tetrathionate Broth Base is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base is used as a selective enrichment for the cultivation of Salmonella spp.
Preparation
- Dissolve 46 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minutes to completely dissolve the medium.
- Cool to 45-50°C and add 20 mL of the Iodine-Potassium Iodide Solution to the prepared Tetrathionate Broth Base. If preparing solution, add 6 grams Iodine + 5 grams Potassium Iodide in 20 mL of purified water.
- DO NOT REHEAT AFTER ADDING IODINE SOLUTION.
Note: Do not add Iodine/Potassium Iodide Solution to tubes until just before inoculation. Chemical Tetrathionate inhibits by oxidation of Thiosulfate through the addition of Iodine just prior to use.
For specifications and data sheets please visit the documents section on the Neogen website here
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