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Description
etrathionate Broth Base is used with iodine for the recovery of Salmonella spp. in a laboratory setting. Tetrathionate Broth Base is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base is used as a selective enrichment for the cultivation of Salmonella spp.
Preparation
- Dissolve 46 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minutes to completely dissolve the medium.
- Cool to 45-50°C and add 20 mL of the Iodine-Potassium Iodide Solution to the prepared Tetrathionate Broth Base. If preparing solution, add 6 grams Iodine + 5 grams Potassium Iodide in 20 mL of purified water.
- DO NOT REHEAT AFTER ADDING IODINE SOLUTION.
Note: Do not add Iodine/Potassium Iodide Solution to tubes until just before inoculation. Chemical Tetrathionate inhibits by oxidation of Thiosulfate through the addition of Iodine just prior to use.
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