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Description
Oxoid™ Sorbitol MacConkey Agar (500g) CM0813B is a selective medium used for the isolation and differentiation of Escherichia coli O157 and other Enterobacteriaceae from clinical, food, and environmental samples. The agar contains sorbitol as a carbohydrate source, which is utilized by most E. coli strains except for E. coli O157, which does not ferment sorbitol and forms colourless colonies.
Key Features:
- Form: Dehydrated powder
- Weight: 500g
- Application: Isolation and differentiation of E. coli O157 from other Enterobacteriaceae
- Composition: Contains sorbitol as a differential agent, bile salts, and crystal violet for selective inhibition of Gram-positive bacteria
- Preparation: Suspend 49.5g in 1 liter of distilled water, mix thoroughly, and autoclave at 121°C for 15 minutes
- Yield: Approximately 10 liters of prepared medium
- Storage: Store the dehydrated medium at 10–30°C, protected from moisture and light
- Compliance: Suitable for use in microbiological testing protocols for the detection of E. coli O157 and other Enterobacteriaceae
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