Oxoid™ Membrane Lactose Glucuronide Agar Pk 10

Detect, enumerate and differentiate Escherichia coli and other coliforms by single membrane filtration with ready-to-use  Oxoid™ Membrane Lactose Glucuronide Agar (MLGA) The presence of lauryl sulphate and lactose with phenol red allow the selective isolation and differentiation of Escherichia coli and coliforms respectively.

Tests for Escherichia coli and coliforms provide a sensitive means for detection of fecal contamination of drinking water. They are regarded as one of the most important routine microbial examinations for assessing the effectiveness of water treatment and disinfection and for monitoring water quality in distribution¹. Membrane Lactose Glucuronide Agar (MLGA) supports water testing utilizing single membrane filtration. 

• Isolates coliforms effectively when used with single membrane filtration method.
• Selectively inhibits Gram-positive organisms, Lauryl sulphate selects for coliforms.
• Reduces confirmatory tests for E. coli due to the specificity of the reactions in the medium.
• Facilitates easy interpretation of the test results due to addition of chromogenic BCIG (5-bromo-4-chloro-3-indolyl-β-D-glucuronide), E. coli appear as green colonies while other coliform bacteria form yellow colonies.
• Ready-to-use convenience of prepared media.

Identification of Escherichia coli and coliforms is facilitated through two biochemical reactions within the medium: Lactose fermentation is detected by the dye phenol red which gives yellow colonies when acid is produced. The chromogenic substrate BCIG is cleaved by the enzyme glucuronidase and produces a blue chromophore which builds up in the bacterial cells. Coliforms are lactose-positive so colonies will be yellow; Escherichia coli is both lactose-positive and possesses glucuronidase so will appear as green colonies.¹

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