Oxoid™ MacConkey Agar (Without Salt) 500g

Oxoid™ MacConkey Agar (Without Salt) (500g) is a modified version of the standard MacConkey Agar, designed for the isolation and differentiation of enteric Gram-negative bacteria, including Escherichia coli and coliforms, without the inclusion of sodium chloride (NaCl). This version is particularly useful when studying bacterial growth patterns without the potential inhibitory effect of salt on certain strains. The medium supports the growth of both lactose and non-lactose fermenters, with lactose fermenters typically producing pink colonies due to acid production, while non-lactose fermenters appear colourless.

Key Features:

• Form: Dehydrated powder
• Weight: 500g
• Application: Isolation and differentiation of Gram-negative enteric bacteria, including coliforms and E. coli, from clinical, food, and environmental samples
• Composition: Contains bile salts to inhibit Gram-positive organisms, lactose for fermentation, and neutral red as a pH indicator. The absence of sodium chloride makes it suitable for specific applications where salt sensitivity may be a factor
• Preparation: Suspend 50g of the dehydrated medium in 1 litre of distilled water, mix thoroughly, and autoclave at 121°C for 15 minutes
• Yield: Approximately 10 litres of prepared medium
• Storage: Store the dehydrated medium at 10–30°C, protected from moisture and light
• Compliance: Suitable for use in microbiological testing protocols for the detection and enumeration of enteric bacteria in food, water, and clinical samples

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