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Description
Oxoid™ Lysine Iron Agar (500g) is a differential medium used primarily for the identification and differentiation of Enterobacteriaceae based on their ability to decarboxylate lysine and produce hydrogen sulphide (H₂S). It is commonly used in diagnostic microbiology to aid in the presumptive identification of organisms such as Salmonella and Citrobacter species from clinical and food samples.
Key Features:
- Form: Dehydrated powder
- Weight: 500g
- Application: Differentiation of Enterobacteriaceae based on lysine decarboxylation and H₂S production
- Composition: Contains lysine for decarboxylation testing, glucose as a fermentable carbohydrate, ferric ammonium citrate and sodium thiosulphate for H₂S detection
- Preparation: Suspend 34g of the dehydrated medium in 1 litre of distilled water, mix thoroughly, and autoclave at 121°C for 15 minutes
- Yield: Approximately 14.7 litres of prepared medium
- Storage: Store the dehydrated medium at 10–30°C, protected from moisture and light
- Compliance: Suitable for use in clinical, food, and environmental microbiology for the differentiation of enteric bacteria
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