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Description
A latex agglutination test for the identification of Escherichia coli serogroup O157.
Certain strains of Escherichia coli have been implicated in cases of haemorrhagic colitis (HC) and haemolytic ureamic syndrome (HUS).
It has been shown that these strains produce a vero-cytotoxin (VT). The Escherichia coli serotype most frequently isolated from HC and HUS case is O157:H7. Isolation of this serotype from a diarrhoeal stool particularly with the presence of blood is indicative of a vero-cytotoxin producing strain.
The Oxoid Escherichia coli O157 Latex Test will demonstrate by slide agglutination Escherichia coli strains possessing the O157 serogroup antigen. The test is best used in conjunction with Sorbitol MacConkey Agar CM813. E.coli O157:H7 strains do not ferment Sorbitol and therefore give colourless colonies on this medium. The majority of Escherichia coli isolates do ferment sorbitol and give characteristic pink colonies.
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