Description
Oxoid™ Clostridium Difficile Agar Base (500g) is a selective medium designed for the isolation and identification of Clostridium difficile from clinical samples, particularly stool specimens. The medium supports the growth of C. difficile while inhibiting the growth of other competing microorganisms. The inclusion of specific ingredients, such as cycloserine and cefoxitin, provides selective pressure to ensure that C. difficile can be differentiated from other bacteria, and the agar base is typically supplemented with additional compounds to enhance the growth of C. difficile and assist in toxin production detection.
Key Features:
- Form: Dehydrated powder
- Weight: 500g
- Application: Isolation and identification of Clostridium difficile from clinical samples, such as stool specimens
- Composition: Contains selective agents like cycloserine and cefoxitin to inhibit the growth of competing organisms, providing an optimal environment for C. difficile growth
- Preparation: Suspend 23g of the dehydrated medium in 1 litre of distilled water, mix thoroughly, and autoclave at 121°C for 15 minutes
- Yield: Approximately 21.7 litres of prepared medium
- Storage: Store the dehydrated medium at 10–30°C, protected from moisture and light
- Compliance: Suitable for use in microbiological testing protocols for the detection and isolation of Clostridium difficile