Mueller Hinton Agar 2 % Glucose w/ Methylene Blue 500g Each

For testing/performing Antifungal Disk Diffusion Susceptibility of yeasts.

The Mueller Hinton formulation was originally developed as a simple, transparent agar medium for the cultivation of pathogenic species (1). Mueller Hinton Agar, Modified (as per CLSI for antifungal) is recommended for the diffusion of antifungal agents impregnated on paper disc through an agar gel as described in CLSI Approved Standard (2). When supplemented with glucose to a final concentration of 2%, it provides for suitable fungal growth. The addition of methylene blue to a final concentration of 5µg/ml enhances zone edge definition.

Kirby-Bauer et al recommended Mueller Hinton Agar for performing antibiotic susceptibility tests using a single disc of high concentration (3). WHO Committee on Standardization of Susceptibility Testing has accepted Mueller Hinton Agar for determining the susceptibility of microorganisms because of its reproducibility (4). Mueller Hinton Agar with 5% sheep blood and Mueller Hinton Agar with Haemoglobin have been recommended for antimicrobial susceptibility testing of Streptococcus pneumoniae and Haemophilus influenzae. Similarly Mueller Hinton Agar, Modified (as per CLSI for antifungal) is recommended for antifungal susceptibity testing of discs.

HM infusion B and Acicase™ provide nitrogenous compounds, carbon, sulphur and other essential nutrients. Starch acts as a protective colloid against toxic substances present in the medium. Starch hydrolysis yields dextrose, which serves as a source of energy. Dextrose (Glucose) serves as an energy source for fungal cultures while Methylene blue enhances zone edge definition.

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