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Description
Muller-Kauffmann Tetrathionate-Novobiocin Broth is used for the selective and differential isolation of Salmonella spp. in a laboratory setting and is included in ISO 6579:2017. This medium is not intended for use in the diagnosis of disease or other conditions in humans. A selective enrichment medium for the detection of Salmonella spp. in food, animal feed and in environmental samples from the food production area as described in ISO 6579-1:2017.
Preparation
- Dissolve 89.4 grams of the medium in one liter of purified water.
- Mix thoroughly and bring to a boil.
- Cool to less than 45°C before adding supplements.
- Supplement NCM0126 with 20 mL of iodine-iodide solution and 4 vials of NCM4040-0.5* Novobiocin. NCM3503 is provided fully supplemented so no further additions are required.
- Mix well and distribute into sterile containers. Iodine-iodide solution Dissolve 25g of potassium iodide in 10 ml of water. Add 20g iodine and dilute to 100ml with sterile deionized water.
For specifications and data sheets please visit the documents section on the Neogen website here
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