MacConkey Broth Purple 500g

MacConkey Broth Purple is used for the detection and enumeration of fecal coliforms (37°C) and E. coli (44°C). This medium is not intended for use in the diagnosis of disease or other conditions in humans. The replacement of neutral red used in the original formulation by bromocresol purple makes the color change caused by acid producing organisms easier to read.

Preparation

1. Dissolve 35 grams of the medium in one liter of purified water.
2. Heat with frequent agitation to completely dissolve the medium if necessary and dispense into tubes or bottles with inverted Durham tubes.
3. Autoclave at 121°C for 15 minutes.
4. Prepare double strength broth (70g/l) if 50ml or 10ml amounts of inoculum are to be added to equal volumes of broth. Prepare single strength broth (35g/l) if 1ml or 0.1ml amounts of inoculum are to be added to 10ml of broth.

For specifications and data sheets please visit the documents section on the Neogen website here