Description
Listeria Chromogenic Agar (according to the formulation of Ottaviani and Agosti) is a selective medium for the isolation and presumptive identification of Listeria monocytogenes from foodstuffs and related materials as described in ISO 11290-1:2017. This medium is not intended for use in the diagnosis of disease or other conditions in humans.
Preparation
- Suspend 70.5 grams of the medium in 950mL of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes.
- Cool to 45-50°C and add 2 vials of reconstituted NCM4002-0.5* supplement, each reconstituted using 5mL of sterile deionized/RO water. Swirl to mix.
- Add 2 vials of NCM4001-0.5* supplement (pre-heated to 48-50°C). Mix well with gentle end-overend mixing.
For specifications and data sheets please visit the documents section on the Neogen website here