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Description
Half Fraser (Demi-Fraser) Broth is used with ferric ammonium citrate for the selective enrichment of Listeria species and is not intended for use mulated according to ISO 11290. The selective components acriflavine and nalidixic acid are blended into the base powder and the ferric ammonium citrate is added to the tempered broth after sterilization.
Preparation
- Dissolve 55 grams of the medium in one liter of purified water.
- Mix thoroughly.
- Autoclave at 121°C for 15 minutes 4. Cool to 45-50°C.
- Aseptically add 2 vials of NCM4009-0.5* Ferric Ammonium Citrate Supplement.
For specifications and data sheets please visit the documents section on the Neogen website here
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