Brain Heart Infusion Agar 500g

Brain Heart Infusion Agar is used for the cultivation of a wide variety of fastidious organisms and is not intended for use in the diagnosis of disease or other conditions in humans. A general purpose nutritious agar base. This medium was first used for the isolation of dental pathogens. With the addition of 7% defibrinated blood the medium will support the growth of a wide range of fastidious organisms, the phosphate buffer will help neutralize the acids produced from the utilization of glucose and thus maintain viability. The medium is not recommended for the determination of hemolytic reactions because of the glucose content.

Preparation

1. Suspend 49 grams of the medium in one liter of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes.

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