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Description
Bacillus Cereus Agar Base is used with Polymyxin B and Egg Yolk Emulsion for the isolation and presumptive identification of Bacillus cereus and is not intended for use in the diagnosis of human disease. The medium is formulated to detect small numbers of B. cereus in the presence of large number of contaminants. This medium differentiates B. cereus from other bacteria based on resistance to polymyxin, lack of mannitol fermentation, and presence of lecithinase.
Preparation
- Suspend 41 grams of the medium in 965 mL of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes.
- Cool to 45 - 50°C. Aseptically add 25 mL of NCM4017* Egg Yolk Emulsion 50% and 2 vials of NCM4032-0.5* Polymyxin B reconstituted using 5 mL sterile deionized/RO water.
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