B12 Culture Agar (E. coli Maintenance Medium) 100g Each

B12 Culture Agar (E.coli Mutant Culture Agar) is recommended for cultivation and maintenance of E.coli ATCC 11105 (1). Tryptone provides nitrogen and amino acids for the growth of E. coli 1133. Yeast extract is vitamin source in the medium while sucrose is carbon and energy source. Potassium salt provides buffering capacity to the medium and sodium, magnesium and ferrous salts control permeability of bacterial cells. HL extract enriches the medium for growth of E. coli as well as inhibits gram positive bacteria.

Stock cultures of E.coli ATCC 11105 are prepared by stab cultures are made at least three times in a week. Do not use the culture for preparing assay inoculum if it is over 4 days old. Before using a fresh culture for assay, make at least 10 successive transfers of the culture in 15 days period. Incubate the cultures for 16-24 hours at 35°C but hold constant within ± 0.5°C. After incubation store at 2-8°C.

Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.

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