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Description
Wort Agar is for the enumeration of yeasts and molds in butter, and is not intended for use in the diagnosis of disease or other conditions in humans. This medium can be modified to enable it to isolate osmophilic yeasts from soft drinks and sugar products by the addition of high concentrations of sucrose and glucose.
Preparation
- Suspend 48.3 grams of the medium in one liter of purified water.
- Add 2.35 mL of glycerol and allow to soak for 10 minutes.
- Autoclave at 121ºC for 15 minutes.
- Use 60 grams per liter if required for inoculation by plate streaking with a wire loop. Do not exceed time or temperature of sterilization.
- If osmophilic modification is required add 48.3 grams of powder to one liter of a solution containing 35% w/v sucrose and 10% w/v glucose then sterilize at 108ºC (5 p.s.i.) for 20 minutes.
For specifications and data sheets please visit the documents section on the Neogen website here
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