Tryptic Soy Agar (TSA) 500g (same as Tryptone)

Tryptic Soy Agar is used for the preparation and maintenance of test strains used in growth promotion tests, suitability of the counting methods and as negative controls as described in the Harmonized USP/EP/JP. It is also a support plating medium for various protocols described in the microbial enumeration test section of the Harmonized USP/EP/JP. Tryptic Soy Agar is not intended for use in the diagnosis of disease or other conditions in humans. Enzymatic digests of casein and soybean act as a source of nitrogen and amino acid and sodium chloride maintains the osmotic balance.

Preparation

1. Suspend 40 grams of the medium in one liter of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes.
4. Cool to 45-50°C.

For specifications and data sheets please visit the documents section on the Neogen website here