Pseudomonas Agar Base 500g (use with either CFC or CN Supp)

Pseudomonas Agar Base is used for the selective and differential isolation of Pseudomonas sp. using CN or CFC supplementation and is not intended for use in the diagnosis of disease or other conditions in humans. 

Preparation

CFC Formula

1. Suspend 48.4 grams of the medium and 10 mL of glycerol in one liter of purified water and bring to a boil.
2. Autoclave at 121°C for 15 minutes.
3. Cool to 45 – 50°C.
4. Aseptically add 2 vials of NCM4023-0.5* CFC Supplement, each reconstituted using 5 mL of 50% ethanol.

CN Formula

1. Suspend 48.4 grams of the medium in one liter of purified water and bring to a boil.
2. Autoclave at 121°C for 15 minutes.
3. Cool to 45 – 50°C.
4. Aseptically add 2 vials of NCM4008-0.5* CN Supplement, each reconstituted using 5 mL of sterile deionized/RO water.

For specifications and data sheets please visit the documents section on the Neogen website here