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Description
Modified Phosphate Buffer is recommended as washing buffer solution required during detection of Escherichia coli O157 : H7 from food and animal feeding stuffs.
The composition and performance criteria are in accordance with ISO 16654: 1999.
This buffer solution is used as washing solution for immunomagnetic separations.
It is important to keep the pH of the suspending fluid within the same range, as variation in pH may affect the viability of the microorganisms.
Phosphate buffer is preferred to unbuffered distilled water in order to standardize wide variation in the pH of distilled water from various sources.
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