Oxoid™ Violet Red Bile Glucose Agar 5kg

Oxoid™ Violet Red Bile Glucose Agar (5kg) is a selective and differential medium primarily used for the isolation and enumeration of coliforms, especially Escherichia coli and other Gram-negative bacteria, from food, water, and environmental samples. The medium is designed to inhibit the growth of Gram-positive organisms while promoting the growth of coliforms, which ferment glucose and produce acid. Coliforms typically form red or pink colonies, making them easy to distinguish from non-fermenters.

Key Features:

• Form: Dehydrated powder
• Weight: 5kg
• Application: Isolation and enumeration of coliform bacteria, particularly E. coli, from food, water, and environmental samples
• Composition: Contains bile salts and crystal violet to inhibit Gram-positive bacteria, glucose for fermentation, and neutral red as a pH indicator to visually distinguish fermenting colonies
• Preparation: Suspend 47g of the dehydrated medium in 1 litre of distilled water, mix thoroughly, and autoclave at 121°C for 15 minutes
• Yield: Approximately 106.4 litres of prepared medium
• Storage: Store the dehydrated medium at 10–30°C, protected from moisture and light
• Compliance: Suitable for use in microbiological testing protocols for the detection of coliforms in water and food