Oxoid™ Simmons Citrate Agar 500g

Oxoid™ Simmons Citrate Agar (500g) is a selective and differential medium used primarily to test the ability of microorganisms, particularly Gram-negative bacteria, to utilize citrate as their sole carbon source. It is commonly used in microbiological research and clinical diagnostics for the identification of enteric bacteria, including members of the Enterobacteriaceae family.

Key Features:

• Form: Dehydrated powder
• Weight: 500g
• Application: Differentiation of bacteria based on their ability to utilize citrate as the sole carbon source
• Composition: Contains sodium citrate as the sole carbon source, ammonium dihydrogen phosphate as the nitrogen source, and bromothymol blue as a pH indicator (color change from green to blue when citrate is utilized)
• Preparation: Suspend 50g in 1 liter of distilled water, mix thoroughly, and autoclave at 121°C for 15 minutes
• Yield: Approximately 10 liters of prepared medium
• Storage: Store the dehydrated medium at 10–30°C, protected from moisture and light
• Compliance: Suitable for microbiological identification and differentiation of Enterobacteriaceae and other Gram-negative bacteria in clinical, research, and food microbiology.

Download SDS