Oxoid™ Rappaport Vassilliadis Soya Peptone Broth (RVS) 500g

Rappaport-Vassiliadis Soya Peptone Broth (Dehydrated) is a selective enrichment medium designed for the isolation of Salmonella spp. from food and environmental samples. The medium takes advantage of specific characteristics of Salmonella that differentiate it from other Enterobacteriaceae, such as:

• Tolerance to high osmotic pressure

• Ability to multiply at lower pH values

• Greater resistance to malachite green

• Less demanding nutritional requirements¹

Applications

Use Rappaport-Vassiliadis Soya Peptone Broth for the selective enrichment of Salmonella species in complex samples containing mixed flora. It is especially suitable for:

• Food testing

• Environmental monitoring

• Fecal samples, without the need for pre-enrichment

Selective Features

• Malachite green, acidic pH, and high magnesium chloride concentration effectively suppress many Gram-positive organisms while favoring the growth of salmonellae.

• Demonstrates superior performance compared to Selenite Enrichment Broth and Tetrathionate Broth for most Salmonella strains, with the exception of Salmonella typhi¹.

Formulation Background

This broth is based on the revised formulation by van Schothorst et al.³, which builds on the earlier work of Rappaport, Vassiliadis, Schothorst, and Renauld⁴. Key modifications to the original formula include:

• Addition of dipotassium hydrogen phosphate for improved buffering, ensuring stable pH during storage of the prepared medium

• Optimized concentration of magnesium chloride hexahydrate (MgCl₂·6H₂O) to enhance selectivity and reliability

These adjustments have been shown to improve the consistency and effectiveness of the enrichment process¹.