Oxoid™ MacConkey Broth Purple 500g

Oxoid™ MacConkey Broth Purple (500g) is a selective and differential medium used for the detection and enumeration of coliforms, particularly Escherichia coli and other enteric Gram-negative bacteria, from water, food, and environmental samples. The medium contains bile salts and crystal violet, which inhibit the growth of Gram-positive bacteria, and is formulated to promote the growth of coliforms. The pH indicator (neutral red) allows for the differentiation of lactose fermenters, with acid-producing bacteria turning the medium pink to red.

Key Features:

• Form: Dehydrated powder
• Weight: 500g
• Application: Detection and enumeration of coliforms, especially E. coli and other enteric Gram-negative bacteria
• Composition: Contains bile salts and crystal violet to inhibit Gram-positive bacteria, lactose for fermentation, and neutral red for pH indicator
• Preparation: Suspend 11.7g of the dehydrated medium in 1 litre of distilled water, mix thoroughly, and autoclave at 121°C for 15 minutes
• Yield: Approximately 42.7 litres of prepared medium
• Storage: Store the dehydrated medium at 10–30°C, protected from moisture and light
• Compliance: Suitable for use in microbiological testing protocols for the detection of coliforms in water, food, and environmental samples