Oxoid™ Half Fraser Broth W/Out Ferric Ammonium Citrate 3kg

Oxoid™ Half Fraser Broth without Ferric Ammonium Citrate (Dehydrated) is a selective secondary enrichment medium designed for the isolation of Listeria species from food and environmental samples. This formulation is a modification of Fraser Broth, containing reduced concentrations of selective agents to aid in the recovery of stressed Listeria cells. The absence of ferric ammonium citrate enhances compatibility with downstream applications such as PCR-based assays and imaging systems.

Key Features:

• Form: Dehydrated powder
• Weight: 3kg
• Application: Secondary enrichment for the detection of Listeria spp. in food and environmental samples
• Composition: Proteose peptone, tryptone, Lab-Lemco powder, yeast extract, sodium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate, aesculin, lithium chloride, nalidixic acid, acriflavine hydrochloride
• Selective Agents: Reduced concentrations of nalidixic acid and acriflavine hydrochloride to facilitate the recovery of stressed cells
• Preparation: Suspend 57.4g in 1 litre of distilled water, mix well, and autoclave at 121°C for 15 minutes. If required, cool to 50°C and aseptically add 10ml of a 5% w/v sterile solution of ferric ammonium citrate
• Yield: Approximately 43.6 litres of prepared medium
• Storage: Store at 10–30°C in a dry environment
• Compliance: Suitable for use in microbiological testing protocols for the detection of Listeria spp.

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