Oxoid™ Blood Agar Base No 2 500g

Oxoid™ Blood Agar Base No. 2 (500g) is a highly nutritious medium used for the cultivation of a wide range of fastidious microorganisms, including bacteria, yeasts, and fungi. This enriched medium is particularly effective for growing pathogens that require the presence of blood for optimal growth. It supports the haemolytic activity of microorganisms, enabling the detection of different types of haemolysis (α, β, and γ) based on the breakdown of red blood cells.

Key Features:

• Form: Dehydrated powder
• Weight: 500g
• Application: Cultivation of fastidious organisms, including pathogens that require blood for growth; useful for the detection of haemolytic activity
• Composition: Contains peptones, sodium chloride, and other nutrients to support bacterial growth; can be supplemented with 5% defibrinated sheep blood for full activity
• Preparation: Suspend 50g of the dehydrated medium in 1 litre of distilled water, mix thoroughly, and autoclave at 121°C for 15 minutes. After cooling, add 5% defibrinated sheep blood if required.
• Yield: Approximately 10 litres of prepared medium (without blood supplement)
• Storage: Store the dehydrated medium at 10–30°C, protected from moisture and light
• Compliance: Suitable for use in microbiological testing protocols for the cultivation and differentiation of haemolytic bacteria

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