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Description
Mueller Hinton Agar II is used in antimicrobial susceptibility testing by the disk diffusion method. This formula conforms to Clinical and Laboratory Standard Institute (CLSI), formerly National Committee for Clinical Laboratory Standards (NCCLS).
Preparation
- Suspend 38 grams of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121ºC for 15 minutes. Cool to 45-50ºC
- OPTIONAL: Supplement as appropriate. Pour cooled Mueller Hinton Agar II into sterile petri dishes on a level, horizontal surface to give uniform depth. Allow to cool to room temperature.
- Check prepared Mueller Hinton Agar to ensure the final pH is 7.3 ± 0.1 at 25ºC.
For specifications and data sheets please visit the documents section on the Neogen website here
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