Legionella BCYE Isolation Medium 500g

BCYE Agar is used (with appropriate supplementation) for the isolation of Legionella spp. from water and environmental samples. BCYE Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Preparation

1. Suspend 38.5 grams of the medium in 1 L of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 110°C for 10 minutes. Cool to 45 - 50°C.
4. Aseptically add 2 vials of NCM4006-0.5* BCYE Growth Supplement, each reconstituted using 5 mL sterile deionized/RO water, to make BCYE agar with Legionella growth supplements.
5. To make GVPC selective medium additionally add 2 vials of NCM4007-0.5* GVPC Selective Supplement, each reconstituted using 10 mL sterile deionized/RO water.
6. Mix thoroughly and continuing mixing throughout dispensing. NOTE: NCM4006 supplement can be substituted with NCM4005 supplement (without the addition of NCM4007 selective supplement) to prepare presumptive identification plates.