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Description
Blood Agar Base is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Blood Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. An inexpensive, general purpose agar base which, with the addition of 5-7% sterile blood, can be used to cultivate a wide range of microorganisms of indicative significance. Typical hemolysis patterns are obtained with this medium.
Preparation
- Suspend 43.1 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes.
- Cool to 45–50°C and add 5-7% sterile defibrinated horse or sheep blood.
- Mix well before dispensing into sterile Petri dishes.
For specifications and data sheets please visit the documents section on the Neogen website here
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