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Description
Bismuth Sulphite Agar is used for the selective isolation of Salmonella spp. In a laboratory setting. Bismuth Sulphite Agar is not intended for use in the diagnosis of disease or other conditions in humans and conforms to FDA/BAM formulation.
Preparation
- Suspend 52 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute.
- Mix thoroughly to obtain a uniform suspension prior to dispensing.
- Freshly prepared Bismuth Sulfite Agar is very selective, therefore suitable for heavily contaminated samples. However, it is not recommended to store prepared Bismuth Sulfite Agar at 4°C for longer than 2 days; after 3 days of storage the medium may change to a green color with a reduction in selectivity of competing microorganisms and an increased potential for the inhibition of Salmonella colonies.
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